1. Circulating mesenchymal stem cells (MSCs), their characterization, gene regulation, releasing and homing mechanisms and applications in tissue repair and regeneration.
We have reported increased numbers of circulating MSCs in patients of long bone fracture, non-union and bone tumors; we have compared and characterized the peripheral blood derived MSCs (PB-MSCs) and bone marrow MSCs and identified several novel genes such as CRBP-1, Aqp-1, Sox-11, Sm51 etc. in governing MSCs migration and osteogenic potentials. We have demonstrated that the systemic administration of MSCs through circulation can enhance tissue repair such as long bone fracture, and has no adverse effects. Circulating MSCs may be a novel cell source for tissue repair and we continue researching on the isolation, culture and further characterization of PB-MSCs and use them as diagnostic, prognostic and therapeutic targets.
PPT: Circulating MSCs and their clincial implications |
Representative Publications:
1.Meng FB, Xu LL, Huang S, Liu Y, Hou YH, Wang KX, Jiang XH, Li G. Small nuclear ribonucleoprotein polypeptide N (Sm51) promotes osteogenic differentiation of bone marrow mesenchymal stem cells by regulating Runx2. Cell & Tissue Research, 2016; in press.
2. Huang S, Xu LL, Zhang YF, Sun YX, Li G. Systemic and local administration of allogeneic bone marrow derived mesenchymal stem cells promotes fracture healing in rats. Cell Transplantation, 2015; 24: 2643–2655.
3. Xu LL, Huang S, Hou YH, Liu Y, Ni M, Meng FB, Wang KX, Rui YF, Jiang XH, Li G. Sox11-modified mesenchymal stem cells (MSCs) accelerate bone fracture healing: Sox11 regulates differentiation and migration of MSCs. FASEB Journal, 2015; 29(4):1143-52.
4. Meng FB, Rui YF, Xu LL, Wan C, Jiang XH, Li G. Aqp1 enhances migration of bone marrow mesenchymal stem cells through regulation of FAK and β-catenin. Stem Cells and Development, 2014; 23(1):66-75.
5. Xu LL, Li G. Circulating mesenchymal stem cells and their clinical implications. Journal of Orthopaedic Translation, 2014; 2: 1-7.
6. Xu LL, Song C, Ni M, Meng, FB, Xie HQ, Li G. Cellular retinol-binding protein 1 (CRBP-1) regulates osteogenenesis and adipogenesis of mesenchymal stem cells through inhibiting RXR-a-induced ß-catenin degradation. The International Journal of Biochemistry & Cell Biology, 2012; 44:612-619.
7. Ma LL, Meng FB, Shi P, Li G, Pang XN. Quantity and proliferation rate of mesenchymal stem cells in human cord blood during gestation. Cell Biology International, 2012; 36:415-418.
8. Bian ZY, Gan YK, Hao YQ, Xu WT, Li G, Tang TT. Increased number of mesenchymal stem cell-like cells in peripheral blood of patients with bone sarcomas. Archives of Medical Research, 2009; 40(3):163-8.
9. He Q, Wan C, Li G. Concise review: multipotent mesenchymal stromal cells in blood. Stem Cells; 2007; 25:69-77.
10. Shirley D, Marsh D, Jordan G, McQuaid S, Li G. Systemic recruitment of osteoblastic cells in fracture healing. Journal of Orthopaedic Research, 2005; 23 (5): 1013-21. |